2.windows ubuntu子系统配置

打开UBuntu后，

> wget https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/Miniconda3-latest-Linux-x86_64.sh #下载conda软件。

> bash Miniconda3-latest-Linux-x86_64.sh #下载完conda后执行这步

> source ~/.bashrc

> conda-h #出现一下结果就成功。
usage: conda [-h] [-v] [--no-plugins] [-V] COMMAND ...

conda is a tool for managing and deploying applications, environments and packages.

options:
  -h, --help          Show this help message and exit.
  -v, --verbose       Can be used multiple times. Once for detailed output, twice for INFO logging, thrice for DEBUG logging, four times for TRACE logging.
  --no-plugins        Disable all plugins that are not built into conda.
  -V, --version       Show the conda version number and exit.

commands:
  The following built-in and plugins subcommands are available.

  COMMAND
    activate          Activate a conda environment.
    clean             Remove unused packages and caches.
    compare           Compare packages between conda environments.
    config            Modify configuration values in .condarc.
    content-trust     Signing and verification tools for Conda
    create            Create a new conda environment from a list of specified packages.
    deactivate        Deactivate the current active conda environment.
    doctor            Display a health report for your environment.
    env               See `conda env --help`.
    info              Display information about current conda install.
    init              Initialize conda for shell interaction.
    install           Install a list of packages into a specified conda environment.
    list              List installed packages in a conda environment.
    notices           Retrieve latest channel notifications.
    package           Create low-level conda packages. (EXPERIMENTAL)
    remove (uninstall)
                      Remove a list of packages from a specified conda environment.
    rename            Rename an existing environment.
    repoquery         Advanced search for repodata.
    run               Run an executable in a conda environment.
    search            Search for packages and display associated information using the MatchSpec format.
    update (upgrade)  Update conda packages to the latest compatible version.

#安装git

sudo apt-get install git

# 创建conda 环境

 conda create -n dna

#激活环境，conda activate dna

#安装软件，我常用的安装软件的方法有两种，基本上大部分软件都可以靠这两种方法安装。

第一种：   conda install -c bioconda fastqc -y   #conda安装软件。

fastqc -h #出现软件参数文档就成功安装，和conda -h 类似。

第二种：下载在本地，在~/.bashrc中输入路径。

比如下载cellranger，

wget -O cellranger-7.2.0.tar.gz "https://cf.10xgenomics.com/releases/cell-exp/cellranger-7.2.0.tar.gz?Expires=1702235980&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=c5ZVKLpCggvKE5O1o0pR7X-IfMXAkWZdQ9o-BipsD9PQJP31eIR2vpy2J2Ucd5faQyM0ZgjsO98qdF616z62D4SRvJDHeYkdQzGSmoZNzcWRLM1Getvend4HkSxPxjePb5MhvohRKsh9~wR2RVivMSZXpiy6zhH1efwcF4-PPmSwJshbhU3pI7InTt5qg92kasL3ekv~8D-scp3kAiFwm15d-ufvblrox8g4P9JQjFHHDjLuu0F~P8-Qc31lMdOe~5KoDCbkE~UI7sEUBJktD0MR~lZ620OcYKgvaqCB-wLgI3VI0rH8RPxYvbgUXryBHnB0wEmqjaessFlNvhql1Q__"。

下载成功后，进入cellranger bin目录，pwd获取绝对路径。

vim ~/.bashrc 

#编辑输入：export PATH="/mnt/h/softwore/cellranger-7.2.0/bin/:$PATH"，保存退出后，

source ~/.bashrc #需要激活。

cellranger -h #出现软件技术说明，就算安装成功。

除此之外，还有其它方法，不过，我暂时没有遇到需求，等到后期，我在分享。